个人简介
翁小成，教授，博士生导师，国家基金委优青获得者，湖北省楚天学者计划楚天学子。2020年湖北省科学技术奖励自然科学一等奖(核酸的分子识别和调控，第2完成人)。2018年获批国家自然科学基金优秀青年基金资助。2015年湖北省楚天学者计划楚天学子。2010年湖北省科学技术奖励自然科学奖一等奖（以核酸为靶目标的小分子的设计、合成及其生物活性研究，第2完成人）。获买球最好的网站第五届青年教师教学竞赛一等奖(2010年)。获英国皇家化学会RSC “Young Chemist Award”参会项目资助(2009年)。已主持国家自然科学基金面上项目4项，青年基金1项，承担国家基金委重大研究计划“基因信息传递过程中非编码RNA的调控作用机制”集成项目课题1项。发表SCI论文80多篇，其中以共同第一/通讯作者在Nat. Chem. Biol.,Cancer Cell,Nat. Commun.,J. Am. Chem. Soc.,Angew. Chem. Int. Ed.等刊物上发表论文30多篇。
教育经历
1998.09-2002.07，买球最好的网站(中国)有限公司，化学基地班，学士
2002.07-2007.11，买球最好的网站(中国)有限公司，有机化学，博士，导师：周翔教授
工作经历
2007.11-2010.11，买球最好的网站(中国)有限公司，讲师
2010.11-2018.09，买球最好的网站(中国)有限公司，副教授
2013.11-2016.11 美国芝加哥大学 访问学者，博士后，导师：何川 教授
2018.09-至今，买球最好的网站(中国)有限公司，教授
研究方向
长期从事核酸化员工物学的研究。基于化学探针等化学技术，融合分子生物学、基因组学、细胞生物学等手段，开发核酸表观遗传修饰、核酸结构以及核酸/蛋白质相互作用的研究技术，并开展相应的功能研究，以及开拓在临床疾病诊断方面的应用。
代表成果
1. Ruitu Lyu, Tong Wu, Allen C. Zhu, Diana C. West-Szymanski,Xiaocheng Weng, Mengjie Chen, Chuan He*. KAS-seq: genome-wide sequencing of single-stranded DNA by N3-kethoxal-assisted labeling.Nature Protocols,2021,accepted.
2. Yafen Wang, Shaoqing Han, Ruoxi Ran, Anling Li, Huanyu Liu, Mingjun Liu, Yongwei Duan, Xiong Zhang, Zhigang Zhao, Shihui Song,Xiaocheng Weng*, Songmei Liu*, Xiang Zhou*, A longitudinal sampling study of transcriptomic and epigenetic profiles in patients with thrombocytopenia syndrome.Nature Communications2021,12, 5629.
3. Wang, Y. F.; Zhang, X.; Han, S. Q.; Yang, W.; Chen, Z. G.; Wu, F.; Liu, J. Z.;Weng, X. C.*; Zhou, X.*, Base-Resolution Analysis of Deoxyuridine at the Genome Scale Based on the Artificial Incorporation Modified Nucleobase.Acs Central Science2021,7(6), 973-979. (封面文章)
4. Wang, Q.; Tan, K. Y.; Wang, H.; Shang, J. H.; Wan, Y. Q.; Liu, X. Q.;Weng, X. C.; Wang, F. A.*, Orthogonal Demethylase-Activated Deoxyribozyme for Intracellular Imaging and Gene Regulation.Journal of the American Chemical Society2021,143 (18), 6895-6904.
5. Wang, B. Y.; Zhang, X.; Wang, Y. F.; Chen, K.; Wang, F.;Weng, X. C.*; Zhou, X.*, One-pot fluorescent assay for sensitive detection of APOBEC3A activity.RSC Chemical Biology2021, 2 (4), 1201-1205.
6. Wang, B. Y.; Chen, Y.; Zhang, X.; Jiang, Z. R.; Wang, Y. F.; Chen, K.; Wang, F.;Weng, X. C.*; Zhou, X.*, A far-red emissive two-photon fluorescent probe for quantification of uracil in genomic DNA.Chemical Communications2021,57 (22), 2784-2787.
7. Li, W.; Yang, H. M.; Wang, Y. F.;Weng, X. C.*; Wang, F.*, Highly sensitive detection of 6mA at single-base resolution based on A-C mismatch.Analyst2021,146 (14), 4450-4453.
8. Huang, J. G.; Zhao, R. Q.; Mo, J.; Wang, F.;Weng, X. C.*; Zhou, X.*, N-3-Kethoxal-Based Bioorthogonal Intracellular RNA Labeling.Chembiochem2021,22 (9), 1559-1562.
9. Zhang, Y. J.; Zhang, Q. Y.;Weng, X. C.; Du, Y. H.*; Zhou, X., NEase-based amplification for detection of miRNA, multiple miRNAs and circRNA.Analytica Chimica Acta2021,1145, 52-58.
10. Huang, J. G.; Zhao, R. Q.; Qin, S. S.; Yang, S. X.;Li, W.; Mo, J.; Wang, F.; Du, Y. H.;Weng, X. C.*; Zhou, X., 4-Thiouridine-Enhanced Peroxidase-Generated Biotinylation of RNA.Chembiochem2021,22, 212-216.
11. Mo, J.; Chen, Z. G.; Qin, S. S.; Li, S.; Liu, C. G.; Zhang, L.; Ran, R. X.; Kong, Y.; Wang, F.; Liu, S. M.; Zhou, Y.; Zhang, X. L.;Weng, X. C.*; Zhou, X.*, TRADES: Targeted RNA Demethylation by SunTag System.Advanced Science2020,7 (19),202001402.
12. Chen, Yuqi; Wu, Fan; Chen, Zonggui; He, Zhiyong; Wei, Qi; Zeng, Weiwu; Chen, Kun; Xiao, Feng; Yuan, Yushu;Weng, Xiaocheng; Zhou, Yu; Zhou, Xiang*, Acrylonitrile-Mediated Nascent RNA Sequencing for Transcriptome-Wide Profiling of Cellular RNA Dynamics.Advanced Science2020,7 (8), 1900997.
13.Weng, X.; Gong, J.; Chen, Y.; Wu, T.; Wang, F.; Yang, S.; Yuan, Y.; Luo, G.; Chen, K.; Hu, L.; Ma, H.; Wang, P.; Zhang, Q. C.*; Zhou, X.*; He, C.*, Keth-seq for transcriptome-wide RNA structure mapping.Nature Chemical Biology2020,16(5), 489-492.
14. Yang, S.; Wu, F.; Peng, S.; Wang, F.; Chen, Y.; Yuan, Y.;Weng, X.*; Zhou, X., A m6A sensing method by its impact on the stability of RNA double helix.Chemistry undefinedamp; Biodiversity2020,https://doi.org/10.1002/cbdv.202000050.
15. Liu, Y. Q.; Zhang, X.; Liu, M.; Xu, F.; Zhang, Q. Y.; Zhang, Y. J.;Weng, X. C.; Liu, S. M.; Du, Y. H.*; Zhou, X.*, Direct detection of circRNA in real samples using reverse transcription-rolling circle amplification.Analytica Chimica Acta2020,1101, 169-175.
16. Zou, G. R.; Liu, C. X.; Fang, Z. T.; Jia, S. K.; Yang, W.; Zhang, X.; Chen, M. K.; Tian, T.;Weng, X. C.; Zhou, X.*, Photocaged probes for spatiotemporal imaging.Sensors and Actuators B-Chemical2019,288, 113-119.
17. Li, W.; Zeng, W. W.; Chen, Y.; Wang, F.; Wu, F.;Weng, X. C.*; Zhou, X.*, Biotinylation and isolation of an RNA G-quadruplex based on its peroxidase-mimicking activity.Analyst2019,144(15), 4472-4476.
18. Li, W.; Wang, F.; Chen, Y.;Weng, X. C.*; Zhou, X., A sensitive and radiolabeling-free method for pseudouridine detection.Analytical Biochemistry2019,581.
19. Chen, Y.; Yang, S.; Peng, S.; Li, W.; Wu, F.; Yao, Q.; Wang, F.;Weng, X.*; Zhou, X.*, N1-methyladenosine detection with CRISPR-Cas13a/C2c2.Chemical Science2019,10, 2975-2979
20. Zhong, X.; Yu, J. Y.; Frazier, K.;Weng, X. C.; Li, Y.; Cham, C. M.; Dolan, K.; Zhu, X. R.; Hubert, N.; Tao, Y.; Lin, F. F.; Martinez-Guryn, K.; Huang, Y.; Wang, T.; Liu, J. Z.; He, C.*; Chang, E. B.*; Leone, V.*, Circadian Clock Regulation of Hepatic Lipid Metabolism by Modulation of m(6)A mRNA Methylation.Cell Reports2018,25(7), 1816-+.
21. Yuan, Y. S.; Hong, T. T.; Chen, Y.; Wang, Y. F.; Qiu, X. P.; Zheng, F.;Weng, X. C.*; Zhou, X.*, Luminescence Sensing for Qualitative and Quantitative Detection of 5-Methylcytosine.Analytical Chemistry2018,90(16), 10064-10068.
22. Wu, F.; Liu, W. T.; Yang, S. X.; Yao, Q.; Chen, Y.;Weng, X. C.*; Zhou, X.*, An aptamer-based ligation-triggered rolling circle amplification strategy for ATP detection and imaging in situ.Journal of Photochemistry and Photobiology a-Chemistry2018,355, 114-119.
23. Wang, Y. F.; Liu, C. X.; Zhang, X.; Yang, W.; Wu, F.; Zou, G. R.;Weng, X. C.*; Zhou, X.*, Gene specific-loci quantitative and single-base resolution analysis of 5-formylcytosine by compound-mediated polymerase chain reaction.Chemical Science2018,9(15), 3723-3728.
24. Wang, Y. F.; Liu, C. X.; Wu, F.; Zhang, X.; Liu, S.; Chen, Z. G.; Zeng, W. W.; Yang, W.; Zhang, X. L.; Zhou, Y.;Weng, X. C.; Wu, Z. G.; Zhou, X.*, Highly Selective 5-Formyluracil Labeling and Genome-wide Mapping Using (2-Benzimidazolyl)Acetonitrile Probe.Iscience2018,9, 423-+.
25. Su, R.; Dong, L.; Li, C.; Nachtergaele, S.; Wunderlich, M.; Qing, Y.; Deng, X.; Wang, Y.;Weng, X.; Hu, C.; Yu, M.; Skibbe, J.; Dai, Q.; Zou, D.; Wu, T.; Yu, K.; Weng, H.; Huang, H.; Ferchen, K.; Qin, X.; Zhang, B.; Qi, J.; Sasaki, A. T.; Plas, D. R.; Bradner, J. E.; Wei, M.; Marcucci, G.; Jiang, X.; Mulloy, J. C.; Jin, J.*; He, C.*; Chen, J.*, R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m(6)A/MYC/CEBPA Signaling.Cell2018,172(1-2), 90-+.
26. Luo, G. Z.; Hao, Z. Y.; Luo, L. Z.; Shen, M. R.; Sparvoli, D.; Zheng, Y. Q.; Zhang, Z. J.;Weng, X. C.; Chen, K.; Cui, Q.; Turkewitz, A. P.; He, C.*, N-6-methyldeoxyadenosine directs nucleosome positioning in Tetrahymena DNA.Genome Biology2018,19.
27. Hong, T. T.; Yuan, Y. S.; Chen, Z. G.; Xi, K.; Wang, T. L.; Xie, Y. L.; He, Z. Y.; Su, H. M.; Zhou, Y.; Tan, Z. J.;Weng, X. C.*; Zhou, X.*, Precise Antibody -Independent m6A Identification via 4SedTTP-Involved and FTO-Assisted Strategy at Single-Nucleotide Resolution.Journal of the American Chemical Society2018,140(18), 5886-5889.
28. Liu, C.; Wang, Y.; Yang, W.; Wu, F.; Zeng, W.; Chen, Z.; Huang, J.; Zou, G.; Zhang, X.; Wang, S.;Weng, X.; Wu, Z.; Zhou, Y.; Zhou, X.*, Fluorogenic labeling and single-base resolution analysis of 5-formylcytosine in DNA.Chemical Science2017,8(11), 7443-7447.
29. Li, Z.; Weng, H.; Su, R.;Weng, X.; Zuo, Z.; Li, C.; Huang, H.; Nachtergaele, S.; Dong, L.; Hu, C.; Qin, X.; Tang, L.; Wang, Y.; Hong, G.-M.; Huang, H.; Wang, X.; Chen, P.; Gurbuxani, S.; Arnovitz, S.; Li, Y.; Li, S.; Strong, J.; Neilly, M. B.; Larson, R. A.; Jiang, X.; Zhang, P.; Jin, J.*; He, C.*; Chen, J.*, FTO Plays an Oncogenic Role in Acute Myeloid Leukemia as a N-6-Methyladenosine RNA Demethylase.Cancer Cell2017,31(1), 127-141.
30. Xu, X.; Mao, W.; Lin, F.; Hu, J.; He, Z.;Weng, X.; Wang, C.-J.; Zhou, X.*, Enantioselective Diels-Alder reactions using a G-triplex DNA-based catalyst.Catalysis Communications2016,74, 16-18.
31. Luo, G.-Z.; Wang, F.;Weng, X.; Chen, K.; Hao, Z.; Yu, M.; Deng, X.; Liu, J.; He, C.*, Characterization of eukaryotic DNA N-6-methyladenine by a highly sensitive restriction enzyme-assisted sequencing.Nature Communications2016,7.
32. Wang, X.; Zhao, B. S.; Roundtree, I. A.; Lu, Z.; Han, D.; Ma, H.;Weng, X.; Chen, K.; Shi, H.; He, C.*, N-6-methyladenosine Modulates Messenger RNA Translation Efficiency.Cell2015,161(6), 1388-1399.
33. Wang, C.-C.; Yan, S.-Y.; Chen, Y.-Q.; Zhou, Y.-M.; Zhong, C.; Guo, P.; Huang, R.;Weng, X.-C.; Zhou, X.*, Triphenylamine pyridine acetonitrile fluorogens with green emission for pH sensing and application in cells.Chinese Chemical Letters2015,26(3), 323-328.
34. Liu, W.; Lai, H.; Huang, R.; Zhao, C.; Wang, Y.;Weng, X.*; Zhou, X.*, DNA methyltransferase activity detection based on fluorescent silver nanocluster hairpin-shaped DNA probe with 5'-C-rich/G-rich-3' tails.Biosensors undefinedamp; Bioelectronics2015,68, 736-740.
35. Fu, Y.; Luo, G.-Z.; Chen, K.; Deng, X.; Yu, M.; Han, D.; Hao, Z.; Liu, J.; Lu, X.; Dore, L. C.;Weng, X.; Ji, Q.; Mets, L.; He, C.*, N-6-Methyldeoxyadenosine Marks Active Transcription Start Sites in Chlamydomonas.Cell2015,161(4), 879-892.
36. Deng, X.; Chen, K.; Luo, G.-Z.;Weng, X.; Ji, Q.; Zhou, T.; He, C.*, Widespread occurrence of N-6-methyladenosine in bacterial mRNA.Nucleic Acids Research2015,43(13), 6557-6567.
